半导体激光和高压静电联合治疗对糖尿病大鼠血浆内皮素和微血管损害的影响

The effects of semiconductor laser irradiation and a high voltage static electric field on small vessel impairment in diabetic rats

目的探讨半导体激光和高压静电治疗对糖尿病大鼠血浆内皮素和血管损害的影响。方法选择健康雄性Wistar大鼠50只,取8只作为正常对照组(正常组),其余大鼠腹腔注射链脲佐菌素(STZ)建立糖尿病动物模型后,随机分为糖尿病对照组(模型组)、半导体激光治疗组(激光组)、高压静电治疗组(高压静电组)、高压静电和半导体激光联合治疗组(联合组)。各治疗组给予相应的治疗,每日1次,10次为1个疗程,治疗2个疗程后取各组大鼠静脉血以及心、肾组织标本,检测其血糖、胰岛素、内皮素(ET)并观察组织病理改变。结果模型组大鼠小血管壁增厚,血糖和ET水平增高,胰岛素活性下降,与正常组比较差异有统计学意义(P<0.01)。激光组、高压静电组和联合组治疗后,ET水平明显下降,与模型组比较差异有统计学意义(P<0.01);联合组的胰岛素水平明显高于模型组,差异有统计学意义(P<0.05)。结论以STZ诱导的糖尿病大鼠出现血糖、胰岛素和ET严重失调,心、肾组织小动脉壁增厚、肾小球水肿以及系膜细胞增生。半导体激光和高压静电治疗对糖代谢紊乱所致的血浆ET水平增高和微小血管损害具有预防治疗作用。

Objective： To study the effects of semiconductor laser irradiation and high voltage static electric fields on small vessel damage in diabetic rats. Methods： Fifty healthy male Wistar rats were randomly divided into two groups： 8 rats in a normal group and 42 in a diabetic model group. The diabetic models were created by intraperitoneal injection of streptozocin. The diabetic model rats were randomly divided into four subgroups： a diabetes group, a semiconductor laser treatment group, a high voltage static electric field treatment group and a comprehensive treatment group receiving combined semiconductor laser and high voltage static electric field exposure. The rats in each treatment group were subjected to the corresponding intervention. After 20 days of treatment, the venous blood, kidney tissue and myocardium tissue of the rats were collected, and the concentrations of blood glucose, insulin, endothelin and tissue were detected. Results： Compared with the normal group, a significant increase in blood glucose and endothelin was observed in the diabetic model group, along with significantly decreased blood insulin and significant small vessel endothelium proliferation in the kidney tissue. Compared with the diabetes group, endothelin levels were significantly lower in all 3 treatment groups, and blood insulin was also higher in the comprehensive treatment group. Conclusion There were severe abnormalities in blood glucose, insulin and endothelin as well as mild impairment of small vessel endothelium proliferation in the diabetic rats. Semiconductor laser and high voltage static electric field exposure have a role in treating and preventing these conditions in diabetic rats.