N-乙酰半胱氨酸对高浓度氧和通气机联合所致大鼠急性肺损伤的防护作用

The protective effects of N-acetylcysteine on hyperoxia and ventilator induced acute lung injury in rats

目的研究N-乙酰半胱氨酸对高浓度氧和通气机联合所致大鼠急性肺损伤的防护作用。方法将36只健康雄性SD大鼠随机分为三组。A组实施空气大潮气量通气,B组实施高氧大潮气量通气,C组为N-乙酰半胱氨酸干预组。每小时行1次动脉血气分析,计算氧合指数(PaO2/FiO2)。测定左肺W/D值,支气管肺泡灌洗液(BALF)白细胞及中性粒细胞计数、TNF-α、IL-1β、MIP-2含量以及肺组织丙二醛(MDA)、超氧化物歧化酶(SOD)水平。结果C组大鼠氧合指数较B组显著增加,左肺W/D值、BALF白细胞及中性粒细胞计数、TNF-α、IL-1β、MIP-2含量、肺组织MDA水平较B组显著下降,而SOD水平较B组显著增加。结论N-乙酰半胱氨酸对高浓度氧和通气机联合所致大鼠急性肺损伤有较明显的防护作用。

Objective To study the protective effects of N-acetylcysteine on hyperoxia and ventilator induced acute lung injury （ALI） in rats. Methods Thirty-six male Sprague-Dawley rats were randomly divided into three groups： group A received large tidal volume with morn air, group B received large tidal volume with 100% 02, group C was interfered with N-acetylcysteine. Arterial blood gases were measured every one hour to calculate oxygenation index （PaO2/FiO2 ）. Wet-to-dry weight ratio （W/D） of left lung was counted. WBCs, neutrophils, TNF-α, IL-1β, and MIP-2 levels in bronchoalveolar lavage fluid （BALF） , malondialdehyde （MDA） and superoxide dismutase （SOD） levels in the lung were assayed respectively. Results PaO2/FiO2 increased significantly in group C than that in group B. W/D of left lung, WBCs, neutrophils, TNF-α, IL-1β, and MIP-2 levels in BALF, MDA levels in the lung were significantly lower, but the levels of SOD signitieandy increased in group C than those in group B. Conclusions N-acetylcysteine has obviously protective effects on hyperoxia and ventilator induced ALl in rats.