Aβ_(1～42)促进体外培养神经干细胞增殖与存活

Proliferative and surviving effect of Aβ_(1～42) on neural stem cells in vitro

目的探索β-淀粉样蛋白1-42(Aβ1～42)对神经干细胞(neural stem cells,NSCs)增殖的影响。方法体外培养新生小鼠神经干细胞,实验分为溶媒对照组、阳性组及Aβ1～420.5、0.1、0.02μmol/L3个剂量组,通过对神经球的形态观察、神经干细胞分化试验、BrdU标记试验、特异性蛋白质免疫细胞化学染色和MTT比色法鉴定NSCs,并以这些指标来判断物质的促NSCs增殖作用。结果在Aβ1～420.1μmol/L剂量组中有大量神经球生成,与对照组有显著差异(P≤0.01),神经干细胞BrdU标记、特异性蛋白质(nestin)免疫细胞化学染色均呈阳性。结论低浓度(0.1μmol/L)Aβ1～42可促进体外培养的NSCs增殖、分裂。

Objective To investigate the effect of amyloid-β protein 1-42 （Aβ1～42） on the proliferation of neural stem cells （NSCs）. Methods The NSCs of newborn mice were cultured in vitro, and treated with 0. 5,0. 1 and 0. 02 βmol/L Aβ1～42,respectively, at the same time, a solvent control and positive drug control were set up to observe neurospheres. MTT assay was used to determine viability of NSCs,and immunocytochemical staining of characteristic protein and BrdU labeling were performed to identify NSCs and their proliferative properties. Results A great deal of neurospheres were formed in the 0. 1 μmol/L group of Aβ1～42 ,differentiating from that in control group （P≤0. 01 ）, in which their characteristic proteins （nestin）of NSCs and BrdU labeling were positive through immunocytochemical stainings. Conclusions The low concentration of Aβ1～42 （0- 1 μmol/L） can promote the proliferation and fission of NSCs in vitro.