喉鳞状细胞癌中细胞外信号调节激酶激活与uPA表达上调的关系

The relationship between the activation of ERK and the up-regulation of uPA in laryngeal squamous cell carcinoma

目的:探讨喉鳞状细胞癌组织中细胞外信号调节激酶(ERK)与尿激酶型纤溶酶原激活剂(uPA)表达间的关系,及其在喉鳞状细胞癌发展中的意义。方法:Western blot法检测40例喉鳞状细胞癌中ERK1/2蛋白的表达,同时检测ERK1/2酶活性以测定其磷酸转移酶效率。免疫组织化学方法检测相应组织中uPA蛋白表达。结合临床相关因素进行统计分析。结果:ERK1/2蛋白表达水平呈线性正相关(P<0.05),与T分级、病理学分级和颈淋巴结状况无关。ERK1/2酶活性表达水平和uPA蛋白表达水平与T分级及病理学分级无关;与颈淋巴结状况有关(P<0.05)。ERK1/2蛋白表达与ERK1/2酶活性表达及uPA蛋白表达无相关性;ERK1/2酶活性表达与uPA蛋白表达呈线性正相关(P<0.05)。结论:ERK激酶过度激活可能通过调控uPA表达上调,促进喉鳞状细胞癌的颈淋巴结转移。

Objective： To study the relationship between the expression of extracellular signal-regulated kinase （ERK） and the expression of urokinase- type plasminogen activator （uPA） as well as its effect on the development of laryngeal squamous cell carcinoma （LSCC）. Method：Western blot was used to detect ERK1/2 protein expression, meanwhile, the change of ERK1/2 activity was examined to measure its efficiency of phosphate transferase in 40 LSCC. Immunohistochemistry was applied to detect uPA protein expression. The correlation of each factor with relevant clinical factor was statistically analyzed. Result： Expression of ERK1/2 showed a positive linear correlation（ P〈0.05）. Neither ERK1 nor ERK2 expression was significantly related to T-grades, histological classes and cervical lymph node status. Neither T-grades, nor histological classes was significantly related to ERK1/2 activity or uPA protein expression. There was significant correlation between ERK1/2 activity and cervical lymph node status and between uPA protein expression and cervical lymph node status（ P〈0.05）. ERK1/2 protein expression was correlated with neither ERK1/2 activity nor uPA protein expression, but there was a significant positive linear association between ERK 1/2 activity and uPA protein expression（ P〈0. 05）. Conclusion： Abnormal activation of ERK may play an important role in cervical lymph node metastasis of LSCC by up-regulation of uPA expression.