心肌肌钙蛋白I异常病人血清结缔组织生长因子表达的研究

Significance of connective tissue growth factor in cardiac troponin I dysfunction patients

目的:检测心肌肌钙蛋白I(cTn I)异常病人血清中结缔组织生长因子(CTGF)水平的变化,探讨CTGF在心肌损伤病人血清中表达的意义。方法:化学发光法检测血清中cTn I水平,并根据cTn I水平分组;利用生物素亲和素EIA分析法检测各组病人血清中CTGF水平。结果:80例病人血清标本按cTn I水平分为Ⅰ、Ⅱ、Ⅲ3组,CTGF平均水平依次为(13.04±6.85)、(21.67±8.35)及(35.06±24.12)ng.m l-1。其中,Ⅱ、Ⅲ组血清CTGF水平明显高于正常对照组(均P<0.05),Ⅰ组与正常对照组比较差异不显著(P>0.05),Ⅱ、Ⅲ组与Ⅰ组比较差异显著(均P<0.05),Ⅱ组与Ⅲ组比较差异显著(P<0.05)。结论:CTGF在心肌损伤以及心肌梗死后表达上调,可能与心肌纤维化关系密切。

Objective To detect the levels of connective tissue growth factor（CTGF） in the sera of cardiac troponin I dysfunction patients and to investigate the meaning of CTGF in patients with myocardial damage. Methods The levelsof cardiac troponin I （ cTn I） were detected by chemoluminescence assay in the sera. According to cTn I concentration, CTGF levels of different groups were also determined using an avidin-biotin-EIA. Results The patients were divided into three groups according to cTn I levels. The average concentrations of CTGF were （ 13.04 ± 6.85 ） ng ·ml^-1 in group Ⅰ ,（21.67 ±8.35）ng ·ml^-1 in group Ⅱ and （35.06 ±24. 12）ng ·ml^-1 in group Ⅲ respectively. CTGF levels of group H and group Ⅲ significantly were increased as compared with control group and group Ⅰ （ all P 〈 0. 05 ）. There was a statistical significance between group H and group Ⅲ （ P 〈 0. 05 ）. However, group Ⅰ had no significance as compared with control group （ P 〉 0. 05 ）. Conclusion CTGF is correlated with myocardial fibrosis after myocardial damage ,or post-myocardiac infarction.