尿脱落细胞中生存素基因表达与膀胱肿瘤的关系

Relationship between Expression of Survivin in Exfoliate Cells and Bladder Cancer Patients

目的:通过检测尿脱落细胞中的生存素(survivin)表达水平,为膀胱癌的临床早期诊断、术后复发监测提供方便、快捷的方法。方法:收集尿脱落细胞、膀胱癌组织和正常膀胱内皮组织,提取其总RNA,采用逆转录-巢式聚合酶链反应(RT-PCR)检测生存素基因mRNA表达水平,同时采用免疫组化进行病理分级。结果:56例膀胱肿瘤患者,尿脱落细胞中生存素mRNA阳性率为96．4％,20例其他泌尿系疾病阳性率为5．0％,10名健康志愿者阴性。膀胱癌组织中生存素基因表达量在1、2、3级闻差异均有极显著意义(P<0．01)。结论:RT-PCR法检测生存素mRNA可以作为早期诊断、术后复发监测的指标。

Objective：To stablish a RT-nest-PCR for diagnosis of bladder cancer through detection of the expression of survivin gene in urine exfoliate cells. Methods： A total of eighty-six urine samples were collected, then the total RNA of exfoliate cells of each sample was extracted, the RT-nest-PCR was established for detection of surviving gene, and the expressive level of survivin gene was checked with immunohistochemistry. Results：Sensitivities of the assay were 96.4% in 56 bladder cancer,5% in other patients and 0% in normal controls. The expression of survivin between G1 ,G2 and G3 bladder cancer tissue had significantly statistical difference（P〈0.01）. Conclusion：Detection of the survivin mRNA by RT-PCR can be used for the early diagnosis, prediction and routine screening for bladder cancer.