泡球蚴组织体外培养的生长发育观察

In vitro Cultivation of Echinococcus multilocularis Metacestodes and Observation of Their Growth

目的观察体外培养泡球蚴的生长发育特性。方法在肝癌细胞系条件下体外培养泡球蚴,用光学显微镜定期观察其数量、大小及形态变化,并绘制囊泡生长曲线。用电子显微镜观察囊泡形态结构。结果体外培养后泡球蚴增多,至22 d其数量相当于开始培养第3～4天的6～7倍;囊泡具有出芽生殖能力。起初均为小囊泡,随时间增加,囊泡的直径也增加,在第22天时约30％为大的囊泡,70％为小的囊泡。发现介于原头蚴与囊泡之间的形态。结论肝癌细胞系体外培养的泡球蚴,生长发育较好且数量增加,可获得纯度较高的分泌或排泄抗原,该方法可为泡球蚴生物学及相关实验研究提供充分实验材料。

Objective To observe the growth and development of Echinococcus multilocularis metacestodes under in vitro cultivation. Methods Hepatoma cell line was used for the cultivation. The number and morphology of the cysts were observed under light microscope. The parasite tissue was fixed and observed under electron microscope. Results During the first 21 days of cultivation, metacestodes in cyst-suspension derived cultures increased dramatically, and from the 22^nd day on, the number of cysts remained as 6-7 times more than that of the 3^rd-4^th day of culture. Budding of new cysts was observed and the diameter of the cysts increased as time went on. On the 22^rd day, larger cysts occupied 30%. Cysts were found with morphology between protoscolex and metacestode. Conclusion An in vitro cultivation for the cysts of E. multilocularis has been established and basic feature of growth and development of the larvae observed.