蒲公英提取物体外抗毛囊蠕形螨活性及皮肤安全性的实验研究

Anti-mite Activity and Skin Safety of Herba taraxaci Extract for Demodex folliculorum

目的探讨蒲公英治疗蠕形螨病的应用价值。方法将粉碎的蒲公英浸泡于80％乙醇中12 h,然后85℃回流提取蒲公英提取液,同法制备百部提取液。取蠕形螨感染者面部皮脂,分离并鉴定蠕形螨备用。设蒲公英实验组,百部对照组和生理盐水对照组,每组蠕形螨15只,进行体外抗螨实验。pH仪测定蒲公英提取物pH值。设蒲公英试验组和75％乙醇对照组,应用健康家兔进行皮肤刺激实验和急性皮肤毒性试验。结果蒲公英提取物的体外杀螨时间为(1．50±0．65)min,显著短于百部提取物的体外杀螨时间(3．53±1．04)min(P<0．01)。生理盐水组螨死亡时间大于120 min。蒲公英提取物的pH值为5．00±0.28,对家兔完整皮肤的刺激评分为0,破损皮肤的刺激评分为0.3,无明显毒性。结论蒲公英提取物具有较强的体外抗毛囊蠕形螨活性且具有皮肤安全性。

Objective To test the in vitro effect of the extract of Herba taraxaci on Oemodex follicldorum. Methods Active Demodex foUiculorum were obtained from patients with moderate to severe demodex infestation. Herba taraxaci and Radix stemonae were extracted respectively with 80% ethanol under 85℃, and a preparation with a concentration of 200mg/ml was made. The extractions were used in vitro to examine the anti-mite activity by observing time of killing mites. Physiological saline and Radix stemonae extraction served as blank control and positive control respectively. PH value of Herba Taraxaci extract was noted. Skin irritation test of normal and wounded skin and acute toxicity test were carried out with rabbit shin, and Herba taraxaci and 75% ethanol were served as experiment and control medicine. Results Motion and morphology of the mites considerably changed with the effect of Herba taraxaci extract. The time of mite-killing was （1.50±0.65）min with Herba taraxaci and （3.53±1.04）min with Radix stemonae respectively （P〈0.01） and over 120 rain with physiological saline, pH value of Herba taraxaci extract was 5.00±0.28. Score for irritation to normal and wounded rabbit skin was 0 and 0.3 respectively, and acute toxicity test showed no significant toxicity. Conclusion Herba taraxaci extract shows a remarkable in vitro activity to Demodex folliculorum with skin safety.