毛细管区带电泳法分离大肠埃希菌定量研究

The quantitative determination of Escherichia coli ATCC25922 by capillary zone electrophoresis

目的:定量研究毛细管区带电泳法(CZE)分离大肠埃希菌(E.coli)以满足临床诊断要求。方法:用毛细管区带电泳法对E.coli ATCC25922进行分离,研究其峰高与E.coli量的关系。结果:毛细管区带电泳法分离E.coli ATCC25922浓度在105cfu/ml～5×107cfu/ml之间线性很好,其峰高与浓度关系符合线性方程:㏑mAU=﹣5.256+1.0036×㏑C,R2=1。结论:CZE法分离大肠埃希菌可进行定量研究。

Objective：A quantitative study ahout determination of Escherichia coli （E.coli）by capillary zone electrophoresis （CZE） was to meet the clinic diagnosis request. Methods：We determined E.coli ATCC25922 by CZE and studied the linear relationship between the peak height （mAU） and the Escherichia coli concentration （C）. Results：The linear relationship of E.coli ATCC25922 concentration determined by CZE between 10^5 fu/ml and 5×10^7cfu/ml was quite good. Regression line equation is cn mAU= - 5.256＋1.0036×cn C, R^2=1.Conclusion： Escherichia coli ATCC25922 determined by capillary zone electrophoresis could be used for a quantitative study.