STZ建立2型糖尿病大鼠模型的剂量探讨

Study on the Best Dosage of Streptozocin in Inducing Rat Model of Type 2 Diabetes Mellitus

目的:探讨链脲佐菌素(streptozocin,STZ)腹腔注射建立2型糖尿病(T2DM)大鼠模型的最佳注射剂量。方法:4周龄SPF级雄性SD大鼠高糖高脂饲料喂养4周,分别按30mg/kg、40mg/kg、50mg/kg体重剂量腹腔注射STZ建立T2DM大鼠模型,观察成模大鼠各阶段体重、食量、饮水量、尿量变化;检测空腹血糖(FBG)、胰岛素(FINS)、血脂,比较各组大鼠模型稳定性。结果:40mg/kg剂量组大鼠的成模率最高(73.3%),成模大鼠具有胰岛素抵抗、高血糖、高胰岛素血症和血脂紊乱的T2DM临床特征,高血糖持续时间最长、最稳定(到实验结束模型稳定至少28d)。30mg/kg剂量组大鼠成模率只有40%,成模大鼠于实验结束血糖水平已基本恢复正常,50mg/kg剂量组成模大鼠倾向于1型糖尿病。结论:短期高糖高脂饮食联合STZ腹腔注射建立2型糖尿病SD大鼠模型的STZ最佳注射剂量为40mg/kg。

Objective To explore the best dosage of streptozocin （STZ） what was used to induce rat model of Type 2 diabetes mellitus （T2DM）. Methods Sixty 4 weeks old male Sprague Dawley （SD） rats were fed with high fatty and high sugar diet for 4 weeks, then STZ （30 mg/kg, 40 mg/kg, 50 mg/kg） was injected intrapefitoneally to make rat model of T2DM, each phase avoirdupois, appetite and the amount of urine and drinking of rat model were tested, in unison, the fasting blood glucose level （FBG）, serum insulin level （FINS） and blood lipid were also detected, the data was used to evaluate the stability of the rat model of T2DM. Results The 40 mg/kg group has the highest making model success rate of over 73.3%, all the succeed model rat has insulin resistance, hyperglycemia, blood lipid disorder and blood insulin increased which were the typical characteristic of T2DM, and duration of high blood glucose was the longest and the most stabile too. Only 40% of the 30 mg/kg group has succeed, beside the blood glucose level has get fight until the end of experimental. The successful rat models of the 50 mg/kg group were inclined to type 1 diabetes mellitus. Conclusion Associate with high fatty and high sugar diet feeding shortly, the best dosage of STZ which was used to induce rat model of T2DM is 40 mg/kg.