二氧化氯颗粒制剂对食源性致病菌的杀菌效果

THE EFFICACY OF GRANULAR PREPARATION OF CHLORINE DIOXIDE IN KILLING FOOD-BORNE PATHOGENIC BACTERIA

目的了解二氧化氯颗粒制剂对食源性致病菌的杀灭效果及影响因素,为其在食品加工领域应用提供依据。方法采用悬液定量杀菌试验方法,对二氧化氯杀灭蜡样杆菌等几种食源性致病菌的效果进行了观察。结果用100 mg/L二氧化氯消毒液作用20 min,对金黄色葡萄球菌平均杀灭对数值可达到6.66。150 mg/L二氧化氯消毒液对蜡样杆菌芽孢作用20 min,平均杀灭对数值达到4.0以上;200 mg/L二氧化氯消毒液作用20 min,可达到完全杀灭。含300 mg/L二氧化氯消毒液对产气荚膜梭菌芽孢作用20 min,杀灭对数值可达到4.0以上;二氧化氯含量增加到350 mg/L作用20 min,可达到完全杀灭。在蜡样杆菌芽孢悬液内加入体积分数10%小牛血清,即可明显降低二氧化氯杀菌效果。二氧化氯对产气荚膜梭菌芽孢杀灭效果浓度效应比较明显,而时间效应只体现在10 min之内。结论二氧化氯对几种食源性致病菌杀灭效果较好,有机物对二氧化氯杀灭细菌芽孢效果影响明显。

Objective To know the efficacy of granular preparation of chlorine dioxide in killing food - borne pathogenic bacteria and its influencing factors in order to provide the basis for its application in food processing area. Methods Suspension quantitative bactericidal test method was used to carry out observation on efffcacy of chlorine dioxide in killing several food -borne pathogenic bacteria such as Bacillus cereus. Results The average killing logarithm value of Staphylococcus aureus exposed to 100 mg/L chlorine dioxide disinfection solution for 20 rain could attain 6.66, that of Bacillus cereus spores exposed to 150 mg/L chlorine dioxide disinfection solution for 20min could attain over 4.0 and the spores could be completely killed after exposure to 200 mg/L chlorine dioxide disinfection solution for 20 min. The killing logarithm value of Clostridium perfringens spores exposed to 300 mg/L chlorine dioxide disinfection solution for 20 min could attain over 4.0 and the spores could be completely killed after exposure to 350 mg/L chlorine dioxide for 20 min. Adding 10% （v/v） calf serum to the suspension orB. cereus spores could reduce significantly the bactericidal efficacy of chlorine dioxide. The concentration effect of chlorine dioxide on efficacy in killing spores of Clostridium perfringens was relatively obvious, while the time effect was shown only within 10min. Condusions Chlorine dioxide has good efficacy in killing several food - borne pathogenic bacteria. Organic substance has significant influence on sporicidal efficacy of chlorine dioxide.