HPLC法测定无味红霉素含量

HPLC determination of erythromycin estolate

建立了离子对高效液相色谱法测定无味红霉素含量。采用C8（5μm，4．0mm×125mm）柱（Lichrospher），以含0．1％（V／V）三乙胺的10mmol／L十二烷基硫酸钠溶液（用磷酸调pH3．4）-乙睛（52：48），检测波长为205nm。线性范围992～4958u／ml，r=0．9998（n=5），回收率100.3％，日内平均RSD为0．6％（n＝18），日间平均RSD为0.8％（n＝9）。

An ion-pair HPLC method was established to determine erythromycinestolate- The liquid chromatograph is equipped with 205nm detector and a 4. 0mm × 125mmcolumn that contains 5 μm packing of Lichrospher C8. The mobile phase is 10mmol/Lsodium lauryl sulfate solution(adjusted with phosphoric acid to pH 3. 4) containing 0.1%(V/V) triethylamine-acetonitrile (52: 48). The linearity is good with r=0. 9998 (n=5) inthe range of 992~4958μg/ml, the recovery is 100. 3%. The RSD within day is 0. 6% (n=18), and the RSD between days is 0. 8% (n=9).