大鼠实验性肝硬变门静脉高压症形成过程中肝组织α_1-受体的放射自显影研究

An autoradiographic study on α_1-adrenoceptors in the hepatic tissues in experimental cirrhotic rats with portal hypertension

用光学放射自显影术对四氯化碳诱导的大鼠肝硬变模型肝组织α_1-受体进行研究,结果发现:正常肝组织结构间α_1-受体密度具有显著性差异(P<0.01);肝硬变各期肝组织结构α_1-受体密度均显著低于正常组的相应组织结构(P<0.01)。本实验组肝内各组织结构α_1-受体的下降可能是四氯化碳的毒性作用和受体下行调节机制等多种原因共同作用的结果。

By using the light autoradiography, the α_1-adrenoceptors in Wistar rats were investigated during the processes of CCl_4-induced cirrhosis with portal hypertension, The α_1-adrenoceptors anr extensively distributed in hepatic tissues of normal rats, and its density varied significantly from one individual structure to another(P<0.01), in an order from high to low; hepatocytes>intrahepatic artery wells>intrahepatic portal walls>intrahepatic venous walls. The density of α_1-adrenoceptors in hepatic tissues in cirrhosis was remarkably lower than that of in normal rats (P<0.01) and the distibution of α_1-adrenoceptors showed no significant difference among different hepatic vascular walls of cirrhosis. The decrease of α_1-adrenoceptors in cirrhotic liver tissues may be caused by the toxic effects of CCl_4 and the downregulation of α_1-adrenoceptors.