摘要
目的:用无血清培养基培养中华仓鼠卵巢(Chinese hamster ovary,CHO)细胞。方法:应用无血清培养基采用小方瓶培养CHO细胞,观察细胞维持时间、培养过程的形态变化。结果:应用无血清培养基培养CHO细胞可维持细胞正常生长。结论:无血清培养基可以完全取代含血清培养基用于培养CHO细胞。
Objective： To culture CHO cells in serum-free media. Methods：Culture CHO cells in serum-free media and then compare the maintainance time,morphological changes and hemagglutination titers of the cells in culture supernatant. Results： The CHO cells cultured in serum-free medium grew well. Conclusion.The serum-containing medium for culture of CHO cells can be completely substituted by serum-free media.
出处
《四川生理科学杂志》
2007年第1期3-6,共4页
Sichuan Journal of Physiological Sciences
基金
国家自然科学基金No.30371307资助
