内脏血管灌注对急性出血坏死性胰腺炎大鼠血浆内毒素含量及肠道细菌移位的影响

Effect of splanchnic vascular infusion on the level of plasma endotoxin and the translocationm of intestinal bacteria in rats with acute hemorrhage necrosis pancreatitis

为观察内脏血管灌注对血浆内毒素含量及肠道细菌移位的影响;以及对肠道超微结构的影响。通过经腹主动脉及门静脉(内脏血管)置管持续交替灌注低剂量多巴胺和低分子右旋糖酐,改善急性出血坏死性胰腺炎(AHNP)时胰腺、肝脏及肠道微循环障碍,结果发现:内脏血管灌注组与未经血管灌注组及周围血管股动脉及股静脉灌注组比较,血浆内毒素含量和肠系膜淋巴结细菌移位率均较低(P<0.01);胰腺及肠道微循环障碍及肠道超傲结构损害也较轻。因此,内脏血管灌注可改善AHNP时胰腺及肠道微循环障碍和降低血浆内毒素含量及减少肠道细菌移位;也说明肠道微循环障碍是AHNP时肠道内毒素及细菌移位的重要因素。

With the aid of the Catheters to abdominal aorta and portal vein (splanchnic blood vessel), we infused low dose dopamine and low molecular weight aextran of the rats with acute hemorrhage necrosis pancreatitis to improve the disorder of microcirculation in pancrease, live and intestinal tract. Four hours after infusion above-mentioned drugs, we obsevered the concentration of plasma endotoxin and the translocation of intestinal bacteria,and the effect on the microcirculation and ultrstructural of pancrease, Liver and intestinal tract. Result: the content of plasma endotoxin and the rate of intestinal bacterial transloca- tion in splanchnie vascular infusion group were lower than that in peripheral blood vessel infusion group and control group. The disorder of microcirculation and the injury of ultrastructure of pancrease, liver and intestinal tract in splanchnic vascular infusion group were minimal in degree. Therefore, splanchnic vascular infusion can improve the disorder of pancrease and intestinal tract,and decrease the level of plasma endotoxin and the rate of intestinal bacterial translocation. That is, the microcirculate disorder of intestinal tract is a important factor of intestinal bacterial translocation and endotoxemia.