慢性乙型肝炎患者HBVDNA与免疫球蛋白、补体的关系

The relationship of HBV DNA with immunoglobulin and complements in patients with chronic hepatitis type B

目的 探讨慢性乙型肝炎患者HBV DNA检出情况与免疫球蛋白、补体的关系。方法 采用荧光定量-聚合酶链反应技术检测120例标本HBV DNA含量，同时速率散射比浊法检测免疫球蛋白IgG、IgA、IgM、补体C3、C4水平。结果 120例标本中，轻度、中度、重度慢性乙型肝炎患者HBV DNA含量差异有统计学意义。与对照组比较，轻、中、重度慢性乙型肝炎患者补体C3水平明显降低，重度慢性乙型肝炎患者补体C4水平也低于对照组；中、重度慢性乙型肝炎患者IgG、IgA含量明显增高，各组IgM差异无统计学意义。结论 FQ—PCR检测HBV DNA具有较高的灵敏度和特异性，结合免疫球蛋白、补体水平的检测结果对乙型肝炎的临床诊断与治疗方案的选择和疗效监测有重要意义。

Objective To investigate the relationship of HBV DNA with immunoglobulin and complements in patients with chronic hepatitis type B. Methods The amount of HBV DNA in the sample of 120 patients were estimated by FQ-PCR. The serum immunoglobulin and complements of these patients were tested with BN Ⅱ. Results The amount of HBV DNA is different in three groups of chronic hepatitis B and control group. The levels of C3 in chronic sever hepatitis B （CSH） and chronic middle hepatitis B （CMH） and chronic slight hepatitis B（CSH） were lower than those of control group. The levels of CA in CSH group were lower than those of control . The levels of IgG and IgA were higher in CSH and CMH group. The levels of IgM did not change in four groups. Conclusion FQ-PCR has high sensitivity and specificity. The amount of HBV DNA with immunoglobulin and complements has an important role in diagnosis of hepatitis B and selection of anti-virus drugs.