油樟叶内生真菌的多样性研究

Diversity of endophytic fungi from leaves of Cinnamomum longepaniculatum N. Chao ex H.W. Li in Yibin,Sichuan,China

采取在马铃薯培养基上加无菌油樟(Cinnamomumlongepaniculatum N.Chao ex H.W.Li)叶汁液作为分离培养基的办法,从油樟树叶中分离到24株内生真菌,经过鉴定,其分属于子囊菌纲的双足囊菌属(Dipo-dascus Lagerh.)、拟指突孢曲霉属(Emericellopsis J.F.H.Beyma)和半知菌类的花核菌属(AnthinaFr.)、青霉属(Penicillium Link)、组丝核菌属(Phacodium Pers.)、茎叶核菌属(Ectostroma Fr.)、地霉(Geotrichum Link)、皮核菌(Acinula Fr.)、团丝核菌(Papulaspora Preuss)、发菌(Capillaria Pers.)、峡串孢霉(Paepalopsis J.G.Khn)、小卵孢霉(Ovularia Sacc.)、卵形孢霉(Oospora Wallr.)、稀丝头孢霉(Haplotrichum Link)、单梗曲霉(Briarea Corda)等15个属,且以无孢菌居多,表现出较为丰富的类群(物种)多样性.

The PDA medium added 0.2 μm filter-sterilized leaf-succus of Cinnamomum longepaniculatum N. Chao ex H. W. Li was used as isolation medium,and twenty four pure endophytie fungal strains were isolated from sudace-sterilized leaves of Cinnamomum longepaniculatum N. Chao ex H. W. Li in Yibin, Siehuan Province, China. These strains were classified into 15 genera： Dipodascus, Emericellopsis, Anthina, PeniciUium, Phacodium, Ectostroma, Geotrichum, Acinula, Papulaspora, CapiUaria, Paepalopsis, Ovularia, Oospora, Haplotrichum ,and Briarea, which showed higher diversity of endophytic fungi in leaves.