尿轻链酶联免疫测定在M蛋白病中的意义

Detecting of urinary free light cbain using ELISA method and its application to monoclonal gammapathies

本研究采用ELISA法测定188例患者尿中轻链κ或λ，结果显示M蛋白病患者尿中轻链呈κ或λ单阳性，肾病、肝病、自身免疫疾病等为轻链双阳性。对M蛋白病轻链分型，其结果与免疫电泳的结果一致，提示采用ELISA法检测患者尿中轻链可作为临床上对M蛋白病的辅助诊断、分型和治疗监测的一项新方法。

An enzyme-linked immunosorbent assay was developed to detect the free light chains (K and X)in urinefrom 188 patients. In the urine from patients with monoclonal gammapathies, either K or A was positive. In the patientswith other diseases, both x and A were positive or negative. The sensitivity, specifity and diagnostic efficiency of theELISA method for monclonal gammapathies were 95. 9%, 95. 2%,and 96. 3% respectively. The P/N of free chains val-ues correlated with the concentration of paraprotein in sera. The results suggested that the ELISA method' may be usedin the diagnosis, classficaticn, and surveillance for monocionai gammapathies.