胺碘酮和维拉帕米对体外激活的淋巴细胞分泌细胞因子的影响

Effects of Amiodarone and Verapamil on the production of cytokine of in intro activated lymphocytes

目的研究胺碘酮(AMI)和维拉帕米(VER)对体外脂多糖(LPS)激活的人外周血淋巴细胞分泌细胞因子的影响,初步探讨此类药物发挥免疫调节作用的机理。方法AMI和VER作用于LPS激活的人外周血淋巴细胞,用双抗体夹心酶联免疫吸附法检测培养液上清中细胞因子白介素2(IL-2)、肿瘤坏死因子α(TNF-α)、白介素10(IL-10)和转化生长因子β1(TGF-β1)水平。结果AMI和VER均可浓度依赖性抑制IL-2、TNF-α和TGF-β1的分泌,提高IL-10的分泌。结论AMI和VER通过对体外淋巴细胞的直接作用调节细胞因子的产生,淋巴细胞膜离子通道可能为其作用靶点之一。

[Objective] To investigate the effects of Amiodarone and Verapamil on the production of cytokine in lipopolysaccharide （LPS）-induced human peripheral blood lymphocytes and to elucidate initially the mechanisms of immunomodulatory action of these agents. [Methods] The isolated human peripheral blood lymphocytes were stimulated by LPS, co-administrated with or without Amiodarone and Verapamil, respectively. The concentrations of cytokines IL-2, TNF-α, IL-10 and TGF-β1 in collected supernate were detected with ELISA. [Resuits] Amiodarone and Verapamil could inhibit the production of proinflammational cytokines IL-2, TNF-α and anti-inflammation cytokine TGF-β1 in LPS-stimulated human peripheral blood lymphocytes and increase the pro- duction of anti-inflammation cytokine IL-10. [Conclusion] Amiodarone andVerapamil influence the production of cytokines through direct pharmaceutical action on lymphocytes. Ion channels in lymphocytes membrane are possibly one of targets of these two agents.