苯巴比妥和地塞米松对鸡细胞色素P450的诱导作用

Induction of phenobarbital and dexamethasone on cytochrome P450 enzymes in chickens

将60只40日龄依莎公鸡随机等分为对照组、苯巴比妥(PB)诱导组和地塞米松(DEX)诱导组,进行体外肝微粒体酶活性测定和体内安替比林(AP)消除动力学试验,研究苯巴比妥和地塞米松对鸡肝微粒体细胞色素P450的诱导作用。结果表明,与对照组相比,PB组和DEX组的肝微粒体酶活性显著提高(P<0.01),AP的代谢速率显著加快(P<0.01)。表明苯巴比妥和地塞米松对鸡肝微粒体体细胞色素P450具有明显的诱导作用。

Sixty 40-day-old cocks were randomly divided into control group,phenobarbital （PB）-treated group and dexamethasone （DEX）-treated group. Induction of phenobarbital and dexamethasone on hepatic cytochrome P450 in chickens was investigated by enzyme assays in vitro and antipyrsine clearance in vivo. The results showed that in comparison with control group the activities of hepatic microsomal enzymes and metabolic rate of antipyrine obviously increased in PB-group and DEX-group （P 〈 0.01）. Microsomal cytochrome P450 enzymes in chickens could be obviously induced by phenobarbital and dexamethasone and the inducible model of cytochrome P450 in vivo was successfully established in this study.