肺炎链球菌新生儿分离株转座酶基因遗传标记的研究

Investigation on the genetic markers of the conjugative transposons in Streptococcus pneumoniae isolated from newborns

目的了解常州地区新生儿呼吸道分离到的肺炎链球菌(Sp)接合型转座子存在状况。方法采用PCR扩增技术对新生儿病房分离到47株Sp菌进行转座酶基因遗传标记——intTn916/Tn1545转座酶基因检测。结果47株Sp菌中39株(83.0%)携带intTn916型或/和Tn1545转座酶基因。结论Tn916型和Tn1545型接合型转座子可携带多种耐药基因,本组Sp菌中intTn916/Tn1545转座酶基因高检出率,揭示了Sp菌获得多重耐药性的遗传学特征。Sp菌通过转座酶基因获得并传播耐药基因,在耐药中起重要作用。

To find out the existence of the conjugative transposons in Streptococcus pneumonoae isolated from newborns in Changzhou, Jiangsu province, the genetic marker intTn916/Tn1545 transposon gene from 47 strains of S. pneumoniae isolated from new horns was investigated by PCR amplification. It was found that 39 stains out of these 47 strains of S. pneumoniae （83.0%） showed the existence of intTn916 or/and intTn1545 conjugative transposon. The high prevalence of these transposons indicates many antibiotics resistance genes are found in the intTN916/intTN1545 conjugative transposon, suggesting that S. pneumoniae could gain the multiple antibiotics resistance via the transfer of transposons.