20(S)-原人参二醇衍生物对HT1080细胞侵袭转移的影响

Effects of 20(S)-Protopanaxadiol derivatives on invasion and migration of HT1080 cells

检测了所合成的20(S)-原人参二醇衍生物GY1和GY2的抗肿瘤活性.GY1对HT1080、SMMC7721、A549的增殖有显著的抑制作用,呈明显的剂量依赖关系,IC50分别为:35.2±0.9 mg/L,29.6±1.1 mg/L,24.1±1.5 mg/L;而GY2并没有表现出的明显的细胞毒作用,其IC50大于100 mg/L.5 mg/L GY1作用48 h可显著降低HT1080细胞对基底膜成分的粘附和侵袭能力,并对细胞的运动能力有一定影响;而相同浓度的GY2虽然对细胞的粘附性和侵袭性有一定影响,但对细胞的运动性却无明显的影响.

The anti-tumor activity of 20（S）-Protopanaxadiol derivatives was detected. GY1 displays growth inhibitory activity against human fibroma sarcomatosum cells HT1080, human hepatoma cells 5MMC7721 and human adenocarcinoma of lung cell A549 in dose dependent. The IC50 values are 35.2 ± 0.9 mg/L, 29.6 ± 1.1 mg/L and 24.1± 1.5 mg/L respectively after 48 hours of GY1 treatment. GY2 does not show obvious cytotoxic activity. 5 mg/L of GY1 can significantly inhibit the adhesion, in- vasion and migration of HT1080 cells. GY2 can also inhibit the adhesion and invasion of HT1080 cells but has no contribution to its mobility in the same concentration.