胃癌组织中COX-2、MMP-9的表达及临床意义

Expression and Significance of COX-2 and MMP-9 in Human Gastric Carcinoma

目的探讨COX-2和MMP-9在人胃癌组织中的表达及临床意义。方法应用免疫组化SABC法检测45例人胃癌组织中COX-2、MMP-9的表达,并以30例正常胃黏膜组织作为对照。对COX-2、MMP-9的表达采用平均光密度测定,并对临床相关资料进行统计学分析。结果胃癌组织中COX-2、MMP-9的平均光密度值(OD)为19.85±7.34,18.81±7.56,显著高于正常胃黏膜组织的表达(12.49±4.87,11.46±4.82,P<0.01)。癌组织中COX-2、MMP-9的表达与患者性别、年龄及肿瘤的部位、组织类型、分化程度无相关性,而与肿瘤直径、TNM分期、淋巴转移、远处转移相关(P<0.05);同时经相关分析发现,在胃癌组织中,COX-2与MMP-9表达有显著相关性(r=0.437,P=0.001)。结论COX-2可能诱导MMP-9的表达而促进肿瘤的侵袭转移。

Objective To study the expression of cyclooxygenase-2 （COX-2） and Matrix Metallo Proteinase-9 （MMP-9） in gastric carcinoma and its possible clinic significance. Methods The expression of COX-2 and MMP-9 were studied by immunohistochemistry SABC method in 45 gastric carcinoma specimen and 30 normal gastric tissues as control. The average light density was assayed by the expression of COX-2 and MMP- 9, and the statistic software was used to analyze the clinic correlated information. Results The average light density（OD） of COX-2 and MMP-9 in gastric carcinoma were 19. 85 ±7. 34,18：81 ±7. 56 respectively,which were significantly higher than those in the normal gastric tissues（ 12. 49 ± 4. 87,11.46 ±4. 82, P 〈0. 01 ）. The expression of COX-2 and MMP-9 were associated with the diameter of tumor,TNM stage,lymph node metastasis and distant metastasis（ P 〈0. 05 ） ,but negatively correlated to age, gender, tumor position, cellular differentiation and histological type. The expression of COX-2 was positively correlated to MMP-9 （ r = 0. 437, P = 0. 001 ）. Conclusion COX-2 may induce the expression of MMP-9 to promote the invasion and the metastasis of gastric carcinoma.