肺癌中细胞S期激酶相关蛋白2的表达及其对预后的影响

Expression of SKP2 protein in lung carcinoma and its implication for prognosis

目的 研究细胞S期激酶相关蛋白2（SKP2）在肺癌中的表达特点及其对肺癌患者预后的影响。方法 利用组织芯片和免疫组化技术，检测SKP2蛋白在89例非小细胞肺癌（NSCLC）、13例小细胞肺癌（SCLC）、5例肺良性肿瘤、5例正常支气管和肺组织中的表达。结果 NSCLC组织中SKP2蛋白阳性率为（23．52±13．57）％，SCLC组织中SKP2蛋白阳性率为（53．85±12．26）％，肺良性肿瘤、正常支气管及肺组织中SKP2蛋白阳性率为（2．91±1．27）％，三者间差异均有统计学意义（P〈0．01）。SKP2蛋白在肺癌中的表达水平与细胞分化程度、淋巴转移及两类肺癌密切相关，差异有统计学意义（P〈0．01），而与患者年龄、性别、TNM分期等无统计学意义（P值均〉0．05）。生存分析显示，SKP2蛋白表达阳性的肺癌患者5年生存率较SKP2表达阴性的肺癌患者降低（P值均〈0．01）。结论 SKP2蛋白在肺癌组织中的阳性表达明显高于肺良性病变或正常肺组织，尤其在SCLC组织中增高显著，SKP2是影响肺癌患者预后的一个独立因素。

Objective To investigate the characteristics of SKP2 protein expression in lung carcinoma tissues and its implication for prognosis. Methods The expression of SKP2 protein was detected in 89 NSCLC,13 SCLC,5 benign lung neoplasms,5 normal bronchus and lung tissues by tissue chip and immunohistochemical techniques. Results The positive rate of SKP2 staining was （ 23.52 ± 13.57 ） % in NSCLC tissues and （53.85 ± 12.26）% in SCLC tissues, significantly higher than （2.91 ± 1.27）% in benign lung neoplasms and normal bronchus and lung tissues. Its expression was highest in SCLC tissues and lowest in benign lung tissues, with a significant difference between them （P 〈 0.01 ）. The expressive level of SKP2 protein in lung carcinoma tissues was closely related to cell differentiation and lymph node metastasis, but not to age, sex, smoking history, tumor site and size, and TNM staging, etc. The survival analysis revealed that the 5-year survival rate of lung carcinoma patients was much lower in SKP2 protein positive expression group than that in negative expression group （ P 〈 0.01 ）. Conclusion The positive expression of SKP2 protein is higher in lung carcinoma than in benign or normal lung tissues, in particular, much higher in SCLC tissue. Moreover, it may be an independent factor to exert negative influence on prognosis of patients with lung carcinoma.