大白菜游离小孢子培养技术研究

Study on Isolated Microspore Culture in Chinese cabbage

游离小孢子培养技术是获得大白菜单倍体植株的有效途径之一。通过对影响大白菜游离小孢子胚胎发生的主要因素—材料基因型、供体母株生长状态、小孢子发育时期、培养方法、培养条件和影响植株再生频率的因素以及小孢子植株的加倍方法等进行了综述。

Isolated Microspore culture is one of the methods to obtain haploid. In this paper, the progress of isolated microspore culture was reviewed：the major factors affecting regeneration; the methods of reduplication; the major factors affecting the formation of embryo, for example, experimental material, culture medium, raise condition, a phase of microspore growth.