不同脱乙酰度壳聚糖对金针菇菌丝体生长代谢的影响

Effect of Chitosan with Different Degree of Deacetylation on the Growth and Metabolism of Mycelium of Flammulina velutipes

壳聚糖是甲壳素经脱乙酰基而得到的高分子阳离子聚合物,具有特殊的分子结构与功能,在科研及应用领域越来越受到人们的重视。以金针菇为材料,研究在不同脱乙酰度(80%、90%、95%)、不同浓度(0.125、0.083、0.063、0.05mg/mL)的壳聚糖的条件下对菌丝体生理生化的影响,结果表明:3种脱乙酰度壳聚糖分别在不同的浓度下,对金针菇菌丝体生长量、蛋白质含量、核酸含量都有明显的促进作用,但随壳聚糖脱乙酰度及稀释倍数不同而有所不同。其中以脱乙酰度为90%,浓度为0.083mg/mL时,促进作用最为明显;但3种不同脱乙酰度不同稀释倍数的壳聚糖对可溶性糖的影响作用表现不明显。

The chitosan is a kind of giant molecule cation polymer that was deacetylated by chitin, and people more and more attention are paid in the research and application area because of its special molecular structure and functions. We took the Flarnmulina velutipes as the material, and studied the physiology and biochemistry effect of the hyphostroma in three kinds of chitosans with different deacetylated degreee（80% ,90% ,95%）,the different concentration（0. 125mg/mL, 0. 083mg/mL,0. 063mg/mL,0. 05mg/mL）chitosan. The results indicated：There kinds of chitosan with different degree of deacetylation promoted the Flammulina velutipes myceliaum growth,protein content, nucleic acid content in different concentration, and the promotion was followed by the degree of deacetylation and the content of chitosan. The most obvious promotion was the chitosan with 90% deacetylation degree and concentration in 0. 083mg/mL. But the effect on soluble sugar was indistinct in there kinds of chitosan with different degree of deacetylation and different concentration.