摘要
瞄准：为了检验脉管的内皮生长的效果，因素(VEGF ) 在胃的癌症房间在试管内的增长上指向了小介入的 RNA (siRNA ) 。方法：几 siRNAs 是进有 Lipofectamine 2000 的人的胃的癌症房间线 SGC-7901 的 transfected。房间不有 LipofectamineTM 2000 的 transfected 或爬(SCR ) siRNA 用作控制。VEGF mRNA 和蛋白质的表示上的 siRNA 的禁止的效果被 RT-PCR 和 ELISA 检测。MTT 试金被用来检验细胞生长的抑制率。在房间对待 siRNA 的房间骑车的变化被流动血细胞计数检测。结果：有效地指向人的 VEGF 的 siRNA 禁止了胃的癌症房间线 SGC-7901 的增长和房间周期的分发。G0/G1 阶段的百分比比在控制房间在 siRNA1-transfected 和 siRNA2-transfected 房间是显著地更高的。VEGF mRNA 的表示显著地在控制房间与那相比在 siRNA1-transfected 和 siRNA2-transfected 房间被禁止。VEGF 蛋白质显著地在 siRNA-transfected 房间减少了，但是没在 SCR siRNA 上有效果。结论：VEGF siRNA 禁止胃的癌症房间在试管内的增长。
AIM： To examine the effects of vascular endothelial growth factor （VEGF）-targeted small interfering RNA （siRNA） on proliferation of gastric cancer cellsin vitro.
METHODS： Several siRNAs were transfected into human gastric cancer cell line SGC-7901 with Lipofectamine 2000. Cells not transfected with LipofectamineTM 2000 or scrambled （SCR） siRNA served as controls. The inhibitory effect of siRNA on the expression of VEGF mRNA and protein was detected by RT-PCR and ELISA. MTT assay was used to examine the inhibition rate of cell growth.The change in cell cycling of siRNA-treated cells was detected by flow cytometry.
RESULTS： siRNA targeting human VEGF effectively inhibited the proliferation of gastric cancer cell lineSGC-7901 and the distribution of cell cycle. The percentage of G0/G1 phase was significantly higher in siRNA1- and siRNA2-transfected cells than in control cells.The expression of VEGF mRNA was significantly inhibited in siRNA1- and siRNA2-transfected cells compared with that in control cells. VEGF protein notably decreased in siRNA-transfected cells, but had no effect on SCR siRNA.
CONCLUSION： VEGF siRNA inhibits proliferation of gastric cancer cells in vitro.
基金
Supported by Science Project of Qingdao Science and Technology Administration, No. 03-1-YN-14
