摘要
瞄准：调查实际上在老鼠胃粘膜的胃的 somatostatin 和促胃液素送信人 RNA 的规定上的 beta-casomorphin-7。方法：Somatostatin 和促胃液素 mRNA 被 RT-PCR 和原位杂交(ISH ) 在 24 只老鼠确定。老鼠被划分成三个治疗组：基础饮食 + 生理盐水(n = 8 ) ，基础饮食 + beta-casomorphin-7 (7.5 乘 10-7 摩尔)(n = 8 ) ，并且基础饮食 +poly-Gly-7 (多样地与 7.5 包含 N 的相等的摩尔 10-7 摩尔 beta-casomorphin-7 )(n = 8 ) 。在为 30 天的口服以后，老鼠被 exsanguinations 打死。结果：在为 30 d 的 beta-casomorphin-7 的 intra 胃的管理以后，促胃液素 mRNA 增加了 52.8%(P 【
0.05， n = 8 ) ，并且 somatostatin mRNA 层次与控制相比在 30.7% 减少了(P 【
0.01， n = 8 ) 。尽管促胃液素 mRNA 表示被 35.6% 与控制组对照提高，在二基因的表达式的有效差量都没在 poly-Gly-treated 组被观察(P = 0.15， n = 8 ) 。一个 casomorphin 答案的长期的口服显著地减少了 D 房间的平灰色，但是在窦和宫底两个都降低 D 房间的数字。有趣地， G 房间的数字在窦和宫底增加了，但是它的平均密度仅仅在窦被扩充。结论：Beta-casomorphin-7 能够从 G 和 D 的规章的肽的 modulating 基因表示。从原位杂交研究的数据显示 beta-casomorphin-7 借助于 somatostatin 的 paracrine 行动间接地影响促胃液素基因表示，并且取决于它的内在的分子的函数。
AIM： To investigate the in vivo effect of beta-casomorphin-7on the regulation of gastric somatostatin and gastrin messenger RNA in rat gastric mucosa.
METHODS： Somatostatin and gastrin mRNA were quantified by RT-PCR and in situ hybridization （ISH）in 24 rats. The rats were divided into three treatment groups： basal diet ＋ physiological saline （n = 8）, basal diet ＋ beta-casomorphin-7 （7.5 × 10^-7 mol） （n = 8）,and basal diet ＋ poly-Gly-7 （containing equal mol of N with 7.5 × 10^-7 mol beta-casomorphin-7） （n = 8）.After oral administration for 30 days, rats were killed by exsanguinations.
RESULTS： After intra-gastric administration of betacasomorphin-7 for 30 d, gastrin mRNA increased by 52.8% （P 〈 0.05, n = 8）, and somatostatin mRNA levels decreased by 30.7% compared with the controls （P 〈0.01, n = 8）. No significant differences in the expression of the two genes were observed in the poly-Gly-treated group, although gastrin mRNA expression was elevated by 35.6% as against the control group （P = 0.15, n =8）. The long-term oral administration of a casomorphin solution significantly decreased the even gray of D-cells,but did not lower the number of D-cells both in the antrum and fundus. Interestingly, the number of G-cells increased in the antrum and fundus, but its average density was augmented only in the antrum.
CONCLUSION： Beta-casomorphin-7 is capable of modulating gene expression of the regulatory peptides from G and D cells. Data from in situ hybridization studies indicate that beta-casomorphin-7 affects gastrin gene expression indirectly by means of the paracrine action of somatostatin, and depends on its intrinsic molecular function.
基金
Supported by the National Natural Science Foundation of China, No. 39770540
