摘要
目的:观察高蛋白饮食及大量蛋白尿对慢性肾功能衰竭(chronic renal failure,CRF)大鼠的加重损害作用。方法:通过先用5/6肾切除大鼠法制作CRF动物模型,成功后再分别给予5/6肾切除大鼠不同蛋白饮食喂养2个月,制作有大量蛋白尿的CRF动物模型。生化检测不同蛋白饮食喂养的5/6肾切除大鼠血尿素氮(blood urea nitrogen,BUN)、血清肌酐(serumcreatinine,Scr)和24 h尿蛋白定量;放免检测血内皮素-1(endothelin-1,ET-1)、血栓素B2(thromboxane B2,TXB2)、6-酮-前列腺素Flα(6 ketone prostaglandin Flα,6-Keto-PGFlα);分光光度计比色法检测血和肾组织超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)水平。结果:高蛋白饮食及大量蛋白尿可通过加重CRF大鼠的血液动力学异常,升高TXB2和ET-1,降低血6-keto-PGFlα;减弱大鼠血SOD和GSH-Px的活性,加速肾损害。结论:高蛋白饮食可通过导致5/6肾切除大鼠大量蛋白尿,影响肾脏血管活性物质表达,降低5/6肾切除大鼠抗氧化能力而加速CRF的进展。
Objective： To study the effects of high-protein diet （HPD） and high-grade proteinuria in aggravating the progression of chronic renal failure （CRF） in rats.
Methods： CRF with high-grade proteinuria was induced by supplying HPD in five sixth nephrectomy rats, and the changes of serum creatinine （Scr）, blood urea nitrogen （BUN）, endothelin-1 （ET-1）, thromboxane B2 （TXB2） and 6-keto-prostaglandin F1α （6-Keto-PGF1α） were observed. At the same time, the content of malondialdehyde （MDA）, and the activities of glutathione peroxidase （GSH-Px） and superoxide dismutase （SOD）were measured in blood and renal tissue of the rats.
Result： HPD and high-grade proteinuria could accelerate the damage of kidney through increasing the levels of ET-1 and TXB2, reducing the level of 6-Keto-PGF1α, and attenuating the activities of SOD and GSH-Px.
Conclusion： HPD can accelerate the damage of kidney through inducing the high-grade proteinuria in five sixth nephrectomy rats, influencing the expression of kidney vasoactive substance, and reducing the antioxidation.
出处
《中西医结合学报》
CAS
2007年第3期333-337,共5页
Journal of Chinese Integrative Medicine
基金
上海市科委重大课题资助项目(No.01DJ19013)
