重庆市江水中有机污染物细胞毒性分析

Analysis on cytotoxicity of organic pollutants in river of Chongqing City

目的了解重庆市主城区嘉陵江和长江水中有机物污染状况,评价水中有机污染物对人群健康的影响。方法固相萃取法萃取水中有机污染物,将萃取的有机污染物溶于二甲基亚砜(DMSO)中,对H4IIE细胞进行染毒,用四甲基偶氮噻唑蓝(MTT)检测水中有机污染物对H4IIE细胞增殖的抑制作用,并用荧光比色法检测水中有机污染物对H4IIE细胞7-乙氧基-3-异吩嗯唑酮.脱乙基酶(EROD)的诱导作用。结果各水样处理H4IIE细胞后,H4IIE细胞的增殖随染毒时间和剂量的增加而呈下降趋势。48和72h处理组200和250倍浓缩的水样对细胞增殖有显著抑制作用(P<0.05,与溶剂对照组比较);2,3,7,8-四氯二苯并-对-二恶英(TCDD)对EROD诱导的半数有效剂量(EC50)值为4.4pg/ml,各水样相对于2,3,7,8-TCDD的毒性当量值分别为(2004.08)大溪沟:2.2×10-4pg/L;(2004.08)寸滩:0.9×10-4pg/L;(2005.01)大溪沟:13.3×10-4pg/L;(2005.01)寸滩:3.7×10-4pg/L。结论污染物对H4IIE细胞的毒性呈现出时间和剂量依赖性;各水样均可诱导H4IIE细胞EROD活性;同一采样点中,枯水期水样毒性大于丰水期水样。

Objective To investigate the organic pollution status of Jialing River and Yangtze River in Chongqing section, and to evaluate the effects of organic pollutants in river water on public health. Method Extracted organic pollutants using solid phase extraction, dissolved the organic pollutants in dimenthy sulphoxide （DMSO）, and the extracts were exposed to the H4IIE cell, used 3 - （4, 5 - dimethylthiazol - 2 - yl）2, 5 - diphenylteyra - zolium bromide （MTT） assay to test the proliferation inhibition of the extracts on the H4IIE cell, and fluorometry was used to test the Ethoxy- Resorufin - O - Deethylase （EROD） inducement of the extracts on the H4IIE cell. Result After exposed to the extracts, the proliferation of H4IIE cell decreased according to the exposure time and dose. The 48h and 72h treatments of 200 × , and 250 × concentrated sampies exhibited significant reductions （P〈 0.05, vs control） to the cell proliferation. The EROD tests showed that the EC50 value for 2, 3, 7, 8 - tetrochlorodibenzo - p - dioxins （TCDD） was 4.4 pg/ml, and the toxic equivalency （TEQ） values of each sample corresponding to 2, 3, 7, 8 - TCDD were 2004.08 Daxigou： 2.2 ×10^-4 pg/L; 2004.08 Cuntan： 0.9 ×10^-4 pg/L;2005.01：Daxigou：13.3 ×10^-4 pg/L;2005.01 Cuntan：3.7 ×10^-4 pg/L respectively. Conclusion The toxicity of organic pollutants to the H4IIE cell showed time and dose dependence;all of the samples could induce the H4IIE cell EROD activity;the toxicity of low water period samples was higher than that of the high water period samples at the same sampling site.