黄绿青霉素对心脏毒性损伤作用

Observation on CIT-induced cardiotoxicity

目的观察黄绿青霉素(CIT)对离体心肌细胞及大鼠心肌组织结构和功能的损伤。方法电镜检查法观察黄绿青霉素致离体心肌细胞超微结构的改变,并对15 mg/(kg.d)CIT喂饲8周的大鼠心肌组织进行病理学观察。结果光镜下CIT处理组大鼠心肌发生变性和坏死,病变呈灶状或条索状分布,有炎性细胞浸润,肌原纤维凝集崩解,部分心肌细胞颗粒变或空泡变性。电镜下,2.5μmol/L CIT组,细胞形状不规则,染色质边集,肌丝明显,胞质内存在脂滴颗粒,线粒体嵴清晰,部分线粒体空泡变性;25μmol/L CIT可使完整的肌细胞结构消失;随剂量增加,毒性作用增强。结论CIT在体内和体外均可引发心肌的变性和坏死。

Objective To observe the effect of citreoviridin（CIT） on cardiac myocytes in vitro and structure and function of myocardium in rats. Methods The CIT - induced ultrastructural changes on primary culture cardiocyte were observed by electron microscopy. The rats were fed with contaminated feed by CIT at 15mg/（kg·d） body weight for 8 week. The hearts were taken away and observed for light microscopy. Results Microscopically, the prominent histopathological changes induced by CIT were manifested as extensive infiltration of lymphocytes and mononuclear cells, myofibril disintegration and cardiac myocyte granular degeneration or vacuolation. The cardiac myocytes were irregular shape, high density chromatin margination occurred under the nuclear membrane, myofilaments and mitochondrial cristae were clear, there was a large amount of lipid droplets in the cytoplasm, some mitochondrial vacuolation appeared in group treated with 2.5μmol/L CIT as examined by electromicroscopy. The intact structure of cardiac myocyte was not seen in group treated with 25μmol/L CIT. The damages of cardiac myocyte were increased with the dose increasing. Conclusions CIT could cause degeneration and necrosis in cardiac myocytes in vitro and myocardium in rats.