中药对镍暴露氧化损伤抑制作用

Effecst of Chinese herbs on free radicals and expression of 8-oxo-dGTPase in cells exposed to nickel

目的研究灌服中药扶正解毒汤（FJD）后兔血清对硫酸镍（NiSO4）暴露的人支气管上皮细胞（16HBE）自由基含量及8-羟基-2-脱氧鸟苷三磷酸酶（8-oxo-dGTPase）表达的影响。方法体外培养的16HBE细胞分别经NiSO4暴露及NiSO4加扶正解毒汤含药血清共同处理后,采用激光扫描共聚焦显微镜（LSCM）及实时荧光定量RT-PCR检测自由基含量及8-oxo-dGTPase表达的变化。结果NiSO4（800μmol/L）暴露的16HBE细胞,其自由基含量及8-oxo-dGTPase表达水平均高于NiSO4与扶正解毒汤（10%）共处理组细胞,P〈0.05~0.01,相对标准偏差（RSD）〉2%。结论8-oxo-dGTPase可作为镍暴露氧化应激的标记物;中药扶正解毒汤通过清除自由基,下调8-oxo-dGTPase的表达,对镍暴露氧化损伤具有显著的抑制作用。

Objective To study the effect of drug contained rabbit serum, prepared by gastric perfusion with fuzheng Jiedu Decoction（FJD）, on the free radicals and expression of 8-oxo-dGTPase in cells exposed to nickel sulfate（NiSO4 ）. Methods After treatment of cultured 16HBE cells with NiSO4 or NiSO4 adding FJD drug serum, the variation of the free radicals and 8-oxo-dGTPase expression were detected by laser scanning confocal microscopy（LSCM） and real-time fluorescence quantitative RT-PCR, respectively. Results The levels of free radicals and the expression of 8-oxo-dGTPase in cells exposed to NiSO4 （800μmol/L） were all higher than that of groups co-treated with NiSO4 and FJD （ P 〈 0.01 - 0.05, RSD 〉 2 % ）. Conclusion The 8-oxo-dGTPase could serve as a useful marker of oxidative stress induced by NiSO4. By scavenging free radicals and down-regulateing the expression of 8-oxo-dGTPase, FJD could have inhibitory effect on oxidative damage induced by nickel.