摘要
GAGE基因通常表达于睾丸组织和部分恶性肿瘤组织中,被认为可能是理想的癌症诊断的标记和治疗的靶位。我们对GAGE基因家族作了生物信息学分析,发现它们在X染色体上串联成簇排列,为灵长类所独有,各拷贝序列趋异度很低。在人类有15个以上的拷贝,在黑猩猩和猕猴分别有3个和4个。对GAGE基因家族构建进化树,并估算了复制事件发生的时间,结果显示在近400万年内陆续发生。用两种方法计算了GAGE各拷贝间的Ka/Ks值,结果为显著大于1,表明该基因家族受到正选择作用。这些结果提示该基因可能与灵长类的特征有关,其在进化上的地位和在配子发育和肿瘤发生中承担的功能值得深入研究。
The gene family GAGE is characterized to be expressed in testis and a portion of tumors, which is considered to be a candidate for diagnostic marker and immunotherapy target. The present study revealed that GAGE gene is unique to primate lineage. At least 15 duplicates with low divergence were found to be clustered in human X chromosome, while chimpanzee and macaque has 3 and 4 in a similar manner. The phylogenefic tree for the duplicates was constructed and the age of the duplication events was estimated, which was ranged from 4 million years ago to present. The Ka/Ks value of the duplicates was significantly greater than 1, indicating that GAGE family is under positive selection. Based on this study, GAGE may contribute to the characteristics of primate species, and the role of GAGE gene in evolution and in the genesis of gametid and tumor deserves further investigation.
出处
《遗传》
CAS
CSCD
北大核心
2007年第5期559-564,共6页
Hereditas（Beijing)
基金
国家自然科学基金(编号:30471906)资助项目~~
关键词
GAGE基因
灵长类
复制
正选择
GAGE gene
primate
duplication
positive selection
