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KLK4 mRNA实时荧光定量检测方法研究 被引量：1

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摘要 应用SYBR Green I作荧光染料,利用磷酸甘油醛脱氢酶(GAPDH)作内对照,建立KLK4 mRNA的实时荧光定量(FQ-PCR)检测方法。结果建立的FQ-PCR方法扩增GAPDH和KLK4 mRNA的效率分别为0.98和0.96;检测GAPDH和KLK4的批内变异系数(CV)分别为0.8%和1.6%,批间CV分别为3.9%和2.4%;基因扩增产物DNA序列分析及熔解曲线分析表明该方法特异可靠。认为本研究建立的FQ-PCR检测方法简便、特异、重复性好、可信度高,可为KLK4基因表达水平的研究提供基础。
作者 于晶 胡成进 闻新棉
机构地区 山东医学高等专科学校 济南军区总医院 济南市第四人民医院
出处 《山东医药》 CAS 北大核心 2007年第11期59-60,共2页 Shandong Medical Journal
关键词 实时荧光定量 PCR KLK4 基因表达
分类号 R655.8 [医药卫生—外科学]
  • 相关文献

参考文献5

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共引文献15

同被引文献6

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引证文献1

山东医药
2007年 第11期

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