RP-HPLC法测定红花中黄酮醇的含量

SEPARATION AND DETERMINATION OF FLAVONO THE FLOWERS OF CARTHAMUS TINCTORIUS BY RP HPLC

用ＲＰＨＰＬＣ法分离并测定了红花中３种黄酮醇类化合物芦丁（Ｉ）、槲皮素（Ｉ）和山萘酚（ＩＩ）。以大豆甙元为内标物，分析柱ＹＷＧＣ１８（２５ｃｍ×４６ｍｍＩＤ，１０μｍ），甲醇—水—磷酸（４８５∶５１５∶０２５，ｐＨ３５）为流动相，流速１０ｍｌ·ｍｉｎ－１，柱温为室温，检测波长３６０ｎｍ，线性范围０１１～０８０μｇ，相关系数γ＝０９９９５～０９９９８，回收率９７８％～９８９％。本法简便、快速、灵敏。

Carthamus tinctorius L. is a kind of traditional Chinese medicine used in the treatment of uterine congestion, cardio vascular disease, thrombosis and so on. Eight flavonoids have been isolated from its flowers. A reversed phase HPLC method for the separation and determination of the three flavonols: rutin(I) , quercetin(II) and kaempferol(III) in the flowers of Carthamus tinctorius L. is described. The column was 25 cm×4 6 mm ID, packed with YWG C 18 (10 μm). The mobile phase was a mixture of MeOH-H 2O-H 3PO 4(48 5∶51 5∶0 25, pH 3 5). The flow rate was 1 0 ml·min -1 . Daidzein was used as an internal standard. UV detection was performed at 360 nm. The relative standard deviation of the method was 1 1～2 6%. The calibration curves showed good linearity over the range of 0 11～0 80 μg, γ=0 9995～0 9998 and detection limits were 2 0～20 0 ng. The recoveries were 97 8%～98 9% for the three flavonols. The method is simple, fast, sensitive and accurate and has been applied to the analysis of Carthamus tinctorius L. of different sources. It can also be used for the quality control of safflower preparations for clinical uses.