用正交试验法对硅胶柱准离子交换色谱溶剂系统的研究

Silica gel column quasi ionexchange chromatography by orthogonal experiment design

目的：探讨硅胶柱准离子交换液相色谱分析生物碱的分离机制、应用原则。方法：采用正交设计直观分析方法进行多因素多水平试验，以不同类型生物碱保留时间为指标，甲醇醋酸醋酸铵不同比例的溶剂系统为流动相进行液相色谱分析。结果：溶剂系统中醋酸铵浓度对生物碱保留时间影响最大，其次是醋酸及甲醇浓度。结论：硅胶柱不但可以用于一般的正相吸附色谱，还可利用其硅胶表面硅醇基进行离子交换色谱分析。据此提出了硅胶柱准离子交换色谱的概念。

OBJECTIVE:To explore the separation mechanism and the usage principle of silica gel column quasi ionexchange liquid chromatography for the analysis of alkaloids.METHODS:Orthogonal experiment was performed to do multifactors and multilevel test,and the retention time of different types of alkaloids was taken as an objective.Subsequently,the liquid chromatography analysis was made by using the mobile phase of different proportions of thanol acetic acid ammonium acetate.RESULTS:The concentration of ammonium acetate in the mobile phase had the greatest effect on retention time,and the next was acetic acid and thanol.CONCLUSION:The silica gel column could be used not only in common positive phase chromatography,but also is the Si OH group at the surface of silica gel for ionexchange analysis.