氧化苦参碱对心肌损伤大鼠心功能保护作用的研究

The Investigation about Protective Mechanism of Oxymatrine on Cardiac Function in Rats with Myocardial Injury

目的:观察氧化苦参碱(Oxymatrine,OMT)对异丙基肾上腺素(Isoproterenol,ISO)致大鼠心肌损伤的保护作用。方法:利用皮下注射ISO诱导大鼠心肌损伤模型,观察OMT对心肌损伤大鼠心脏功能的影响,并测定各组大鼠血浆乳酸脱氢酶(LDH)的活力、丙二醛(MDA)的含量和超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)的活性。结果:与正常对照组比较,ISO组大鼠LVESP降低30．8%(P＜0．01)、LVEDP增加145．8%(P＜0．05)、±LVdp/dt_(max)分别降低37．4%、40．1%(均P＜0．01),且血浆LDH、MDA的含量分别增高45．5%、23．8%(均P＜0．01),SOD、GSH-PX的活力分别下降14．8%、12．4%(P＜0．05);与ISO组比较,OMT低高剂量治疗组均可明显减轻心内膜下心肌缺血性损伤,并改善心肌损伤大鼠的心功能。结论:氧化苦参碱可通过抑制心肌脂质过氧化、增强抗氧化能力,从而改善心肌损伤大鼠的心功能,对损伤心肌产生保护作用。

Objective： To observe the protective effects of oxymatrine on myocardial injury in rats induced by Isoproterenol （ISO）. Methods： The myocardial injury model was induced by subcutaneous injection of ISO, to observe the effect of oxymatrine on cardiac function in myocardial injury in rats, the lactic dehydrogenase （LDH） activity, the malondialdehyde（MDA） content, the superoxide dismutase （SOD） and Glutathione peroxidase （GSH-PX） activities in plasma were measured in different groups. Results： Compared with normal control group, left ventricular end systolic pressure （LVESP） of the ISO group was decreased 30.8% （P〈0.01） , left ventricular end diastolic pressure （LVEDP） was increased 145.8 % （P〈0.05） , maximal velocity for left ventricular pressure rising and declining （±Lvdp/d tmax） were decreased 37.4%, 40.1% respectively （P〈0.01） , besides the contents of LDH, MDA in plasma were increased 45.5%, 23.8% respectively （P〈0.01） , the activites of SOD, GSH-PX were decreased 14.8%, 12.4% respectively （P〈0.05）; compared with ISO group, groups therapied with low-dosage and high-dosage oxymatrine can siganJficantly ameliorated the myocardial ischemia injury and improve the cardiac function. Conclusion： oxymatrine had cardioprotective effects on cardiac function in myocardial injury rats by inhibiting lipid preoxidation, enhancing anti-oxidation ability.