阴沟肠杆菌Ampc酶的分离纯化

Purification of AmpC β-lastamase in Enterobacter Cloacae

目的:分离纯化Ampc酶并对酶的理化特性进行相关研究。方法:运用离子层析等技术对酶分离纯化;运用电泳等技术研究理化特性。结果:获得纯品酶并证实为Ampc酶。结论:本实验所采用的分离纯化技术可获得纯品Ampc酶。

Objective： To purify AmpC β-1astamase and study its characterizations. Methods： AmpC β-1astamase was purified by the techniques of ion-exchangers, and its molecular weight and isoelectric point were studied by electrophoresis. Results： The purified AmpC β-1astamase can be acquired and proved. Conclusion： The methods of Purification of AmpC β-1astamase might be acquired.