热应激对雄鼠生殖器官及精子发生的影响

Effects of heat stress on spermatogenesis

每天在固定时间用不同梯度温度(42℃,37℃和33℃)处理实验组鼠1 h,热应激前后分别记录小鼠的肛温和体重。在应激的第8 d、13 d分别处死受试鼠后分离睾丸、附睾并分别称量其重量,计算睾丸、附睾与体重指数,将睾丸和附睾组织切片后镜检。结果表明,应激组小鼠的肛温显著升高(P<0.05);睾丸和附睾内的精子数量、生精细胞上皮层数显著减少,精母细胞数量减少,且排列混乱,细胞的凋亡数量增多;而睾丸、附睾与体重指数无明显变化。

Eighty four mice were divided into 4 groups and the experimental groups of mice were treated at 42℃, 37℃ and 33℃ for 1h daily, respectively, in the fixed time. The anus temperature and body weight were recorded before and after the heat stress. The testicle and epididymis were weighted. The index of the testicle, epididymis and the body weight were calculated. The sections of testicle and epididymis were made and examined under a light microscope. The results indicated that the anus temperature of the mice in the experimental group was significantly elevated （P 〈 0.05 ）; The number of spermatozoon, spermatocyte and sperm cell epidermis layer all reduced, and the number of apoptosis cells increased. However, there was no significant change in the index of the testicle, epididymis and the body weight.