慢性胃炎患儿胃窦组织促胃液素和生长抑素及增殖细胞核抗原与凋亡基因配体水平表达

Expression of Gastrin,Somatostatin,Proliferating Cell Nuclear Antigen and Fas-Ligand in Mucosa of Gastric Antrum in Children with Chronic Gastritis

目的探讨慢性胃炎患儿胃窦黏膜组织促胃液素(GAS)、生长抑素(SS)、增殖细胞核抗原(PCNA)、凋亡基因配体(Fas-L)的表达在发病机制中的意义。方法50例胃窦黏膜标本采用快速尿素酶试验及Giemsa染色法诊断,其中慢性胃炎幽门螺杆菌(Hp)阳性20例(A组),慢性胃炎Hp阴性19例(B组);胃窦黏膜未见明显病变11例为对照组(C组)。采用免疫组织化学EnVision法对每例组织分别进行GAS、SS、PCNA、Fas-L染色。结果GAS、SS的表达A组和B组均高于C组,但无显著性差异;PCNA表达A组显著高于B组(P=0.019);Fas-L表达各组间无显著差异。结论慢性胃炎患儿GAS、SS表达均有增高,GAS和SS升高可能在小儿慢性胃炎发病因素、防御机制中起一定作用;Hp感染促进胃窦黏膜上皮细胞增殖。

Objective To investigate the expression of immunohistochemistry of gastrin（GAS） ,somatostatin（ SS）, proliferating cell nuclear antigen（PCNA） and Fas - ligand（ Fas - L） in the sinus ventriculi of children with pediatric gastritis and to explore the significance of their expression in the pathogenesis of pediatric chronic gastritis. Methods Fifty cases of the sinus ventriculi mucosa samples were enrolled in 3 groups：chronic gastritis,helicobacter pylori（Hp） positive（group A,n =20） ;chronic gastritis, Hp negative（group B,n = 19） ;control group, normal sinus ventrlculi mucosa, Hp negative （ group C, n = 11 ）, Immunohistochemistry En Vision were carried out including GAS, SS, PCNA and Fas - L, Results In the expression of GAS and SS, the values of group A and B were comparatively higher than those of group C, but there was no significant difference among them in statistics, In the expression of PCNA, the value of group A was comparatively higher and that of group B, The value difference between 2 groups was significant （P = 0.019） ;in the expression of Fas - L, no significant difference was found among these 3 groups. Conclusions Expressions of GAS and SS both increase in children with chronic gastritis and maybe the increase of GAS and SS play a role in the pathogenesis of pediatric chronic gastritis;Hp infection promotes the multiplication of the sinus ventriculi membrana mucosa epithelium cell in pediatric chronic gastritis.