幼鼠肠易激综合征模型肠道微生态的对照研究

Observation and Analysis of Intestinal Flora Microecology in Infant Rats Model with Irritable Bowel Syndrome

目的探讨幼鼠肠易激综合征(IBS)模型肠道微生态变化情况。方法建立幼鼠IBS模型,共20只,随机选10只用培菲康治疗为治疗组A,另10只为阳性对照组B,取同期正常生长幼鼠10只为阴性对照组C。分别对3组幼鼠行粪便直接涂片革兰染色及菌群检测,同时对鼠粪便干湿重及粪便含水量进行比较。结果幼鼠IBS模型组(A+B)肠道菌群失常率达70%;C组为30%。模型组与C组比较有显著差异(χ2=4.34P<0.05)。3组鼠粪便干湿重及粪便含水量比较,B组与A、C组间有显著差异(Pa<0.05)。结论IBS与肠道菌群改变有一定相关性,药物治疗IBS幼鼠对其粪便等临床症状的改善有一定作用,临床上加用肠道微生态制剂有一定的理论依据。

Objective To explore the changed status of the intestinal flora microecology in the model infant rats with irritable bowel syndrome（IBS） by integrative method. Methods To establish 20 infant rats model with IBS. The IBS rats were randomly divided into the treatment group A with bifico and positive control group B,and another 10 infant rats which grew up regularly were taken as negative control group C, The feces smear staining and intestinal flora detection was performed in the 3 groups respectively,meanwhile the dry and wet weight of each group rats feces and the content of water in the feces were compared. Results The intestinal flora imbalance rate of infant rats IBS group（ A ＋ B） was 70%, and there was 30% in the control group C, the difference between model group and group C was very significantly（X^2 = 4.34 P 〈 0.05 ）. The dry and wet weight of the 3 groups＇ feces and the content of water in the feces were compared, and the difference between group B and A,C groups was very significant（ Pa 〈 0.05 ）. Conclusions A certain correlation exists between IBS and intestinal flora imbalance. The drug treatment can improve the clinical symptoms and signs of the IBS infantile rats. So there is a rational reason for intestinal microecological products being applied in clinical practice.