弥散性毒性甲状腺肿患儿血清白细胞介素-10、12水平变化的意义

Changes of Serum Interleukin-10 and Interleukin-12 in Children with Graves Disease and Its Clinical Significance

目的观察弥散性毒性甲状腺肿(GD)儿童在疾病不同阶段血清IL-10、12水平变化的临床意义。方法采用酶联免疫吸附法(ELISA)测定GD患儿疾病不同阶段(新发病、治疗未缓解、治疗缓解3个阶段)血清IL-10、12水平。检测游离T3、T4(FT3、FT4),敏感TSH(sTSH),甲状腺微粒体抗体(TMAb)、甲状腺球蛋白抗体(TGAb)、甲状腺受体抗体(TRAb)、甲状腺刺激抗体(TSI)水平并分析其间的关系。结果IL-10、12在GD新发病组均显著高于未缓解组、缓解组和健康对照组(Pa<0.05)。IL-12与sTSH呈显著负相关(r=-0.309 P<0.05),IL-10与FT4、FT3呈显著正相关(r=0.350,0.334 Pa<0.05)。结论IL-10、12均参与GD的发病,可作为病情监测的指标。

Objective To study the levels of interleukin -10（IL -10） and IL-12 in serum of patients with Graves disease（GD） in different stage of disease and its clinical significance. Methods The concentrations of serum IL - 10 and IL - 12 in GD in different stages of disease （preliminary diagnosed, unrelieved ,relieved） were measured by enzyme- linked immunosorbent assay （ELISA） and compared with the level of thyroid function ： free tetraiodothyronine （ FT4 ）, free triiodothyronine （ FT3 ）, sensitive thyrotropic - stimulating hormones （sTSH） and thyroid microsomal antibody（ TMAb ）, thyroglobulin antibody （ TGAb ）, thyrotrophin receptor antibody （ TRAb ） and thyroid - stimulating immunoglobulins （TSI）. Results IL - 10 and IL - 12 levels were significantly higher in the patients preliminary diagnosed than other three groups （ Pa 〈 0.05 ）, both of them were found to be related with diseases changes. IL - 10 level in patients with Hashimoto＇s thyroiditis（HT） was negatively correlated with sTSH （ r = - 0. 309 P 〈 0. 05 ）. IL - 10 level in patients with HT was positively correlated with FT3 and FT4 （ r = 0. 350,0. 334 Pa 〈 0. 05）. Conclusion Both IL - 12 and IL - 10 are involved in GD and can be signs of disease changing.