脂肪组织来源干细胞复合胶原构建组织工程皮肤修复皮肤缺损

Construction of tissue engineered skin by adipose tissue-derived stem cells and collagen for skin defect repair

目的:探讨脂肪组织来源干细胞(ADSC)作为皮肤组织工程种子细胞修复皮肤缺损的可能性。方法:采用胶原酶消化SD大鼠脂肪组织,经体外扩增后接种于胶原凝胶构建组织工程活性皮肤替代物。15只SD大鼠随机分成3组:ADSC复合胶原组织工程活性皮肤替代物实验组、单纯胶原移植组和空白对照组。将各组相应材料分别移植于SD大鼠背部皮肤缺损处,比较观察创面修复效果。结果:ADSC复合胶原实验组、单纯胶原移植组和空白对照组创面愈合时间分别为:(14.3±1.7),(16.9±2.5)和(21.2±4.2)d,差异有显著性意义(P<0.05)。组织学观察显示,ADSC复合胶原实验组成纤维细胞、微血管、胶原纤维均较单纯胶原移植组和空白对照组明显增多。结论:ADSC复合胶原构建的组织工程活性皮肤替代物移植后可加速新生血管形成、促进创面修复。提示以ADSCs作为种子细胞复合胶原构建的组织工程皮肤可用于皮肤缺损的修复治疗。

Objective：To investigate the feasibility of adipose tissue-derived stem cells（ADSCs） as the seeding cells of skin engineering for skin defect repair. Methods ： Adipose tissues were isolated from a SD rat groin, the fat was cut and incubated in a collagenase solution, and then the ADSCs were cultivated in culture medium. The amplified cells in vitro were inoculated in collagen gel to form tissue engineered skin. Fifteen SD rats were randomly divided into three groups： experimental group was treated with ADSCs collagen tissue engineered active skin substitute, and single collagen and blank were served as two eontrol groups. The materials were grafted onto total skin defect wounds on the back of rats in the corresponding group respectively. The effect of wound repair was observed histologically. Results：The time of wound healing in the ADSCs and eollagen experimental group, the single collagen control group and the blank control group were （ 14.3 ± 1.7） days, （ 16.9 ± 2.5 ） days, and （ 21.2 ± 4.2 ） days respectively, and showed significant differenees（ P 〈0.05 ）. Histological observation showed that the fibroblasts, micro-vascutarities and collagen fibers in the ADSCs and collagen experimental group were signifieantly increased as compared with those in single collagen group or in the blank control group. Conclusion ： ADSCs with collagen can accelerate the formation of fresh blood vessels of the wounds and promote the wound repair. It suggests that ADSCs with collagen maybe an optional method of skin reconstruction for wound repair.