α1β3、ICAM-1、E-cadherin、P-选择素对宫颈癌局部浸润作用的研究

The study of effection about α1β3,ICAM-1,E-cadherin and P-selectin on local invasion of cervical carcinoma.

目的探讨细胞黏附分子在宫颈癌局部浸润中的作用。方法应用组织芯片技术和免疫组织化学方法对比检测子宫颈鳞状细胞原位癌30例、微小浸润癌34例、浸润癌36例中α1β3、ICAM-1、E-cadherin和P-选择素的表达。结果α1β3在原位癌中的表达率明显低于微小浸润癌和浸润癌(P<0.05),微小浸润癌与浸润癌的表达率无显著差别(P>0.05);P-选择素的表达率在原位癌、微小浸润癌和浸润癌中无显著差别(P>0.05);ICAM-1、E-cadherin在原位癌中的阳性表达率均显著高于微小浸润癌(P<0.05),微小浸润癌中的阳性表达率均显著高于浸润癌(P<0.05)。ICAM-1、E-cadherin在微小浸润癌、浸润癌中的表达水平密切相关(P<0.01),ICAM-1、E-cadherin的阳性表达与子宫颈癌的浸润呈显著负相关(r=-0.8726,P<0.01和r=-0.7968,P<0.01)。结论α1β3、ICAM-1、E-cadherin与宫颈癌的局部浸润有关,其中ICAM-1、E-cadherin在宫颈癌局部浸润过程中可能起着重要作用。

Obejective：To explore the effect of cell adhesion molecules on local invasion of cervical carcinoma. Method ：The expression of proteins α1β3, ICAM-1, E-cadherin and P-selectin were detected by immmunohistochemistry method and tissue microarray technology in 30 cases of carcinoma in situ, 34 cases of microinvasive carcinoma and 36 cases of invasive carcinoma. Results.The expression ratio of α1β3 in carcinoma in situ was apperantly lower than that of microinvasive carcinoma and invasive carcinoma（P 〈0.05）. There was no difference in α1β3 expression between microinvasive carcinoma and invasive carcinoma （P 〉 0. 05 ）. There was no difference in P-selectin among carcinoma in situ, microinvasive carcinoma and invasive carcinoma（ P 〉 0.05 ） ; Both the expression ratio of ICAM-1 and E-cadherin in carcinoma in situ were higher than that of microinvasive carcinoma（ P 〈 0.05 ）, and the expression ratio of ICAM- 1 and E-cadherin in microinvasive carcinoma were （ P 〈 0.05 ）. There was positive correlation both higher than that of invasive carcinoma between the expression of ICAM-1 and E-cadherin in invasive carcinoma and microinvisive carcinoma. There was nagitive correlation between the expresssion of ICAM-1, E-cadherin and the invision in cervical carcinoma （ r = - 0. 8726, P 〈 0.01 and r = - 0. 7968, P 〈 0.01 ）. Conclusion： α1β3, ICAM-1, E-cadherin are related to the invasion of cervical carcinoma. Decreased expression of ICAM-1 and E-cadherin may play a key role in invasion of cervical carcinoma.