一株产白藜芦醇真菌的分离及培养

Study on the Isolation and Culture of a Resveratrol-producing Fungus

在虎杖组织培养过程中分离出1株真菌,对其发酵液提取物进行TLC和HPLC分析证明,该菌株能产生白藜芦醇,并对其培养条件及白藜芦醇积累特性进行了初步研究。结果表明,培养时间、温度、pH值、接种量对白藜芦醇积累均有较大影响。通过单因素和正交试验得到最佳培养条件为:20g/L蔗糖、2g/LNH4NO3、自然pH值(6.8～7.0)、温度为28℃、培养时间7d、接种量为培养基装液量的10%,转速100r/min,其白藜芦醇含量约为39.58μg/L。

A fungus was separated from the tissue cultural processes of Polygonurn cuspidaturn and the zymotic extracts were analyzed by TLC and HPLC. We found that the fungus which is B-39 can produce resveratrol . The cultural optimum conditions and the resveratrol-accumulating characteristics were studied and the results showed that the cultural time, temperature, pH and inoculums had more influence among other factors. The optimization of cultural substrates and conditions were suggested by one- factor and orthogonal tests that the optimum cultural substrates were： saccharine 20 g/L, NH4NO4 2 g/L , the optimum cultural conditions were - pH 6.8-7.0 ,28 ℃, 7 d, initial inoculums 10 %, rotational speed 100 r/min and the content of resveratrol in the fungus was determined as 39.58μg/L by HPLC.