大鼠急性肾脏缺血再灌注后处理动物模型的建立

Postconditioning animal modeling of acute kidney ischemia reperfusion in rats

目的:建立肾脏缺血后处理动物模型.方法:将40只SD大鼠随机分为对照组、缺血再灌注组(IR组)和3个缺血后处理组(IPO1,IPO2,IPO3组),分别制作动物模型.IR组在同时夹闭双侧肾动脉45min后恢复血供,IPO1,IPO2和IPO3组在肾缺血45min后分别采用不同的后处理方法,其中IPO3组采用反复10次再灌注20s-缺血20s的后处理方法.恢复血供24h后留取各组大鼠静脉血标本及肾组织,检测肾功能指标,光镜下观察肾组织形态学变化并对肾小管损伤程度进行评分.结果:IPO3组血尿素氮为(27.9±3.2)mmol/L,血肌酐为(232±49)μmol/L,肾小管损伤程度评分为382±48.和IR组相比,IPO3组的血尿素氮、血肌酐及肾小管损伤程度评分均明显降低(P<0.01),肾组织损伤明显减轻,其余两个缺血后处理组与IR组相比差异无统计学意义(P>0.05).结论:在急性肾脏缺血后,采用IPO3组的方法可以减轻急性肾脏缺血再灌注损伤,从而成功建立大鼠急性肾脏IRI的后处理动物模型.

AIM： To investigate the establishment of rat model of ischemic postconditioning based on the model of acute kidney ischemia reperfusion injury （IRI）. METHODS： 40 SD rats were randomized into 5 groups： control group, ischemia reperfusion group （ IR group） and 3 ischemic postconditioning groups （ IP01, IP02, IP03 groups）. IR group was induced by clamping the bilateral renal arteries for 45 rain. IP01, IP02, IP03 groups were treated by different postconditioning methods after 45 min kidney ischemia. The postconditioning method of IP03 group was sequential 20 sec reperfusion and another 20 sec ischemia for a 10 cycles. After 24 h reperfusion, the blood samples were draw from the inferior vena cava and the nephridial tissues were fixed. Then renal function was detected by biochemical methods, morphologi- cal changes and tubular injury score of nephridial tissues were examined by PAS staining under light microscope. RESULTS： The blood urea nitrogen level of IPO3 group was （ 27.9 _＋ 3.2 ） mmol/L, the serum creatinine level was （232 ± 49 ） μmol/L, and the tubular injury score was （382 ± 48）. Compared with the IR group, the above 3 indexes of the IPO3 group were decreased remarkably （ P 〈 0.01 ） , and the injury of kidney was reduced significantly. However, there was no significant difference between the IR group and the other 2 ischemic postconditioning groups （ P 〉 0. 05 ）. CONCLUSION： The postconditioning method of the IP03 group after the acute kidney ischemia can reduce acute kidney IRI and help us to establish the postcondi- tioning model of the acute kidney IRI in rats.