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可溶性人PD-1IgV(shPD-1IgV)活性检测及抑瘤作用的观察

Bioactivity and antitumor effect of shPD-1IgV
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摘要 目的:检测可溶性人PD-1IgV(shPD-1IgV)的活性,观察其抑瘤效果.方法:利用Ni-NTA亲和层析和分子筛层析纯化蛋白,对其进行复性得到可溶性人PD-1IgV(shPD-1IgV).通过结合ELISA和竞争ELISA检测shPD-1IgV生物学活性,计算抑瘤率检测其抑瘤效果.结果:高效气相色谱分析结果表明纯化复性的shPD-1IgV蛋白的纯度达到95%以上;shPD-1IgV能够分别与鼠源性和人源性B7H1/Fc结合;其与人源性B7H1/Fc的结合可被抗人B7H1mAb阻断;治疗组抑瘤效果与对照组比较具有统计学意义(P<0.05).结论:纯化复性的可溶性人sPD-1IgV具有很高的纯度和生物学活性,体内显示了良好的抑瘤效果. AIM： To characterise the bioactivity of the soluble human programmed death-1 IgV （ shPD-1IgV ） and observe the antitumor effect of the shPD-1IgV. METHODS： The affinity chromatography and gel-filtration chromatography purified and renatured the shPD-1IgV. The bioactivity of the shPD-1IgV was identified by binding ELISA and competitive ELISA. The anti- tumor effect of the shPD-11gV was observed by the tumor inhibi- tion rate. RESULTS： The purity of the shPD-11gV was more than 95% by HPLC. The purified shPD-1IgV could be associated with the mouce and human B7H1/Fc respectivly. Meanwhile, the association of shPD-1IgV with human B7H1/Fc could be competed by the anti-human B7H1 mAb. There was significant difference between therepy group and control group in antitumor （ P 〈 0.05 ）. CONCLUSION ： The purified and renatured shPD- 11gV had a high purity and bioactivity in vitro. In vivo, the shPD- 1IgV could inhibit effectively the tumor growth.
作者 吴守振 王伟华 韩苇 张英起
机构地区 第四军医大学药学系生物技术中心
出处 《第四军医大学学报》 北大核心 2007年第9期815-818,共4页 Journal of the Fourth Military Medical University
关键词 shPD-1IgV 复性 生物活性 肿瘤 治疗 shPD-1IgV renaturation bioactivity tumor therapy
分类号 Q786 [生物学—分子生物学]
  • 相关文献

参考文献11

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二级参考文献10

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第四军医大学学报
2007年 第9期

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