鲈鱼和小鼠肝组织几种功能指标的比较

Comparative study on functional indices of liver in largemouth bass(Micropterus salmoides) and mice

为探索处于不同进化地位的两种动物(鲈鱼和小鼠)肝功能指标的异同,分别采用Lowry法、CO还原差示光谱法和比色法,对鲈鱼和小鼠肝组织重要细胞器(线粒体和微粒体)蛋白、药物代谢酶(细胞色素P450)和反映肝功能的重要指标(血清中天冬氨酸转氨酶和丙氨酸氨基转移酶含量)进行了比较。结果显示,鲈鱼肝脏线粒体蛋白、细胞色素P450和丙氨酸氨基转移酶均明显低于小鼠,而微粒体蛋白和天冬氨酸氨基转移酶与小鼠差异不明显。这些结果提示,肝功能指标在不同进化地位的动物(鲈鱼和小鼠)间存在着差异,其中肝脏细胞色素P450和丙氨酸氨基转移酶的含量可能与肝功能差异密切相关。

The important organelles protein （mitochondria and microsome ）, cytochromes P450 of drugmetabolizing enzymes as well as aspartate transaminase （AST） and alanine transaminase （ALT） in serum of important liver functional indices were comparatively analyzed by Lowry protein measure, CO difference spectrum and colorimetric method to explore the difference of liver functional indices between largemouth bass and mice from different evolution status. Results showed that the level of mitochondria protein, cytochromes P450 as well as ALT in largemouth bass liver were significantly lower compared to mice. The level of microsome protein and AST in largemouth bass and mice liver were not significantly different. These data suggest that liver functional indices of largemouth bass and mice from different evolution status are significantly different, the functions of liver are closely correlated with the content of cytochromes P450 as well as ALT in liver.