副溶血弧菌对南美白对虾生理生化指标的影响

The effects of physiological and biochemical index for Penaeus vannmei infected with Vibrio parahaemolyticus

在自然条件下从患红体病的养殖南美白对虾肝脏内分离出病原菌——副溶血弧菌,将该菌通过肌肉注射的方式,对南美白对虾做感染实验,以探讨副溶血弧菌对南美白对虾生理生化指标的影响。注射弧菌后南美白对虾死亡率与时间、浓度呈正相关,与半致死剂量呈负相关;虾体肌肉、肝脏组织的超氧化物歧化酶(SOD)、溶菌酶(LSZ)酶活性随弧菌浓度的增加呈下降的趋势,而过氧化物酶(POD)酶活性呈上升趋势,这可能是应激反应;酯酶同工酶(EST)酶带表现出缺失,苹果酸脱氢酶同工酶(MDH)具有明显的组织特异性,并且两种酶在肌肉组织中表达最强。副溶菌弧菌感染后破坏了虾体的免疫系统,导致免疫机能的损坏,防病、抗病能力下降,进一步导致虾体患病,甚至死亡。

The effects of physiological and biochemical index for white-leg shrimps（Penaeus vannmei）infected with Vibrio parahaemolyticus were studied in this paper. It is a kind of bacteria - Vibrio parahaemolyticus that was isolated from hepatopancreas of diseased shrimps farmed under natural conditions. The infection experiment that healthy shrimp muscles were injected with the isolated bacteria - Vibrio parahaemolyticus was carried out. After injection shrimp mortality was positive interrelationship to duration time and the injection concentration of Vibrio parahaemolyticus, and was negative interrelationship to semilethal concentration （LD50）. The tests showed that the activity of SOD and LSZ in muscle and hepatopancreas of shrimp declined, but activity of POD was ascendant with increase of injection concentration of Vibrio parahaemolyticus. The EST bands showed absence. Tissue specific of MDH was very distinct, the expressions of EST and MDH in muscle were most strong among examined tissues. After injection of bacterial - Vibrio parahaemolyticusimmunity system of shrimp was destroyed that resulted in immunity function damaged, the abilities of preventing disease and disease resistance declined, shrimps suffered from illness, even died.