阿霉素明胶微球的制备及对周围神经阻滞的实验研究

Preparation of adriamycin gelatin microballoon and its effect on blocking the conduction of peripheral nerves

目的制备阿霉素明胶微球并检测其特性,探讨将其用于周围神经阻滞的可行性。方法采用乳化-交联法制备阿霉素明胶微球,考察其理化性状。30只雄性SD大鼠随机均分为对照组(N组)、阿霉素组(A组)、阿霉素明胶微球组(M组),于右侧坐骨神经表面分别滴注0.1%吐温盐水、0.5%阿霉素、阿霉素明胶微球混悬液。观察每组右后肢的痛阈、坐骨神经功能指数(SFI)及病理改变。结果制备的阿霉素明胶微球最佳投料比为1∶10。微球外形圆整,分散性好。阿霉素240min释放90%以上。A组和M组痛阈明显升高(P<0.01),SFI明显减小(P<0.01),A组60天恢复,M组持续90天仍未恢复,两组坐骨神经损毁明显。结论制备的阿霉素明胶微球外观好,有明显缓释作用,能损毁周围神经,可作为长效神经损毁剂。

Objective To prepare adriamycin gelatin microballoons and identify its characteristics, and to study the feasibility of utilizing the microballoon to block the conduction of peripheral nerves. Methods Adriamycin was embedded in gelatin by means of emulsifycross link, and its physical cbemical property was tested. Thirty male SD rats were randomly divided into 3 groups including control group （group N）, adriamycin group （group A） and adriamycin gelatin microballoon group （group M）. 0. 1% Tween saline, 0. 5% adriamycin and the suspension of adriamycin gelatin microballoons were dropped separately onto the surface of denuded right sciatic nerve. The pain threshold of right hind limb, sciatic nerve function index （SFI） and pathological change were observed. Result The optimal proportion of drug and carrier was 1：10. The microballoon was round in shape with perfect dispersibility. 90 % of the active ingredient could be released from the microballoons within 240 minutes. The pain thresholds in both groups A and M were elevated obviously, and the value of SFI was decreased markedly. These phenomena lasted only 60 days in group A, whereas over 90 days in group M. Conclusions The adriamycin gelatin microballoons possess a good appearance and a satisfactory performance of delayed release. It can damage eperipheral nerve, and may be used as a long acting neurolytic agent.